Solid State Fermentation (SSF) – Protocol, Applications, Advantages, Limitations − BiologyGate.com

Solid-state fermentation (SSF) is a common technique for the production of microbial metabolites. The process of SSF is performed on a solid substrate with a low moisture content, with the advantages of a high product concentration but only a relatively low energy being required. The required water content in SSF is absorbed by the substrate in a solid matrix and offers more advantages for the growth of microorganism for the transfer of oxygen. Several agricultural wastes, such as rice straw, sugarcane bagasse, wheat straw, rice hulls, and corn cobs, are being used as substrate for SSF. Industrially, metabolites production is mostly carried out under submerged fermentation (SmF) conditions due to the simpler downstreaming process of SmF compared to SSF. In the case of SmF, there is a possibility of strong and simple control over the various parameters, whereas there is a knowledge gap in the SSF process. Although SmF is an easier process in terms of operating parameters than SSF, the microbial mycelial morphology is better suited for microbial secondary metabolites production in SSF. Thus SSF exhibits several advantages, like easy gaseous transportation, less water consumption, use of cellulosic waste, pH control, and smaller fermenters are required for SSF and thus less effort is needed for downstream processing. However, SSF technology is needed to be explored in order to improve production of metabolites at a large scale.

During the last 18 years, much research have been devoted either in the form of research/review articles or books on SSF and the recovery of microbial metabolites, due to the fact that this is a well-suited process of microbial metabolism for the production of various value-added metabolites. Apart from this, pollution causing solid wastes, which are rich source of cellulose, protein may be utilized as substrates for SSF. Specifically, Japan is frequently using SSF for the production of various products such as enzymes, amylase, protease, and soya sauce. Further, much SSF-related equipment has been designed and adapted well in Japan for the production of various industrially important microbial metabolites. Additionally, in India Biocon Company started the SSF process for enzyme production more than 20 years ago. Though SSF is an easy process to conduct and has several advantages, the process still suffers from certain drawbacks which need to be overcome for large-scale processing. It has been noted that SSF is slower than SmF with difficult downstreaming properties. Moreover, the SSF process also has difficulties with heat dissipation, which can be limited by inter- and intraparticle conflicts and hence are uncontrolled because of the absence of satisfactory sensors and supportive solid handling techniques for continuous operations. In addition, various issues such as an efficient process to improve SSF, bioreactor design, and sustainability are some critical limiting factors for SSF which need to be tailored based on the existing advancements in SSF technique.

Solid State Fermentation (SSF) is a fermentation method used by several industries like the pharmaceuticals, food, textile etc., to produce metabolites of microorganisms using solid support in place of the liquid medium. It is defined as the growth of microbes without free-flowing aqueous phase. The SSF is alternative to submerged fermentation for production of value-added products like antibiotics, single cell protein, PUFA’s, enzymes, organic acids, biopesticides, biofuel and aroma production. The support used is especially grain brans, de-oiled oil seed cakes, and other substances alike. Initially, mostly fungi were used in such fermentation (as these microorganisms were considered to be very optimally active in very low water activity). Later, many bacterial species and yeasts were used to carry out such fermentation also. The microbiological process of SSF has generated great interest in recent years because it can be used for a variety of purposes, supported by some authors who have even indicated numerous advantages over their liquid counterparts (submerged fermentation).

Solid State Fermentation (SSF) Substrate

There are many biotechnological processes that involve the growth of organisms on solid substrates in the absence or near absence of free water.

Solid state fermentation (SSF) deals with substrates that are solid and contain low moisture levels.

The most regularly used solid substrates are cereal grains (rice, wheat, barley, and corn), legume seeds, wheat bran, lignocellulose materials such as straws, sawdust or wood shavings, and a wide range of plant and animal materials.

Most of these compounds are polymeric molecules – insoluble or sparingly soluble in water – but most are cheap and easily obtainable and represent a concentrated source of nutrients for microbial growth.

SSF can be defined in terms of the following properties:

A solid porous matrix which can be biodegradable or not, but with a large surface area per unit volume, in the range of 103 to 106 m2/ cm3, for a ready microbial growth on the solid/gas interface.

The matrix should absorb water amounting to one or several times its dry weight with a relatively high water activity on the solid/gas interface in order to allow high rates of biochemical processes.

Air mixture of oxygen with other gases and aerosols should flow under relatively low pressure and mix the fermenting mash.

The solid/gas interface should be a good habitat for the fast development of specific cultures of molds, yeasts or bacteria, either in pure or mixed cultures.

The mechanical properties of the solid matrix should stand compression or gentle stirring, as required for a given fermentation process. This requires small granular or fibrous particles, which do not tend to break or stick to each other.

The solid matrix should not be contaminated by inhibitors of microbial activities and should be able to absorb or contain available microbial foodstuffs such as carbohydrates (cellulose, starch, sugars) nitrogen sources (ammonia, urea, peptides) and mineral salts.

Organisms Used in Solid State Fermentation (SSF)

The microbiological components of SSF can occur as single pure cultures, mixed identifiable cultures or totally mixed indigenous microorganisms.

Some SSF processes e.g., tempeh and ontjom production, requires selective growth of organisms such as molds that need low moisture levels to carry out fermentation with the help of extracellular enzymes secreted by fermenting microorganisms.

However, bacteria and yeasts, which require higher moisture content for efficient fermentation, can also be used for SSF, but with a lower yield.

Steps in Solid State Fermentation (SSF)

SSF is normally multistep processes involving the following steps:

1. Pre-treatment of substrate raw materials either by mechanical, chemical or biochemical processing to enhance the availability of the bound nutrients and also to reduce the size of the components, e.g., pulverizing straw and shredding vegetable materials to optimize the physical aspects of the process. However, the cost of pre-treatment must be balanced with the eventual product value.

2. Hydrolysis of primarily polymeric substrates, e.g., polysaccharides and proteins.

3. Utilization (fermentation) of hydrolysis products.

4. Separation and purification of end products.

The low moisture content of SSF enables a smaller reactor volume per substrate mass than LSF and also simplifies product recovery.

However, serious problems arise with respect to mixing, heat exchange, oxygen transfer, moisture control and gradients of pH, nutrient and product as a consequence of the heterogeneity of the culture.

The latter characteristic of SSF renders the measurement and control of the above-mentioned parameters difficult, laborious and often inaccurate, thereby limiting the industrial potential of this technology. Due to these problems, the micro-organisms that have been selected for SSF are more tolerant to a wide range of cultivation conditions.

Applications of Solid State Fermentation (SSF)

Solid-state fermentation has emerged as a potential technology for the production of microbial products such as feed, fuel, food, industrial chemicals, and pharmaceutical products.

It is widely applied to producing several enzymes, organic acids, flavoring compounds etc., which must be extracted and purified and then used in different products.

Its application in bioprocesses such as bioleaching, bio-beneficiation, bioremediation, bio-pulping, etc. has offered several advantages.

Advantages of Solid State Fermentation (SSF)

The main advantage of such methods is that it produces a minimum amount of waste and liquid effluent thus not very damaging to the environment.

Solid substrate fermentation employs simple natural solids as the media.

Low technology, low energy expenditure and requires less capital investment.

No need for sterilization, less microbial contamination, and easy downstream processing.

Utilization of agro-industrial residues as substrates in SSF processes provides an alternative avenue and value-addition to these otherwise under- or non-utilized residues.

The yield of the products is reasonably high.

Bioreactor design, aeration process, and effluent treatment are quite simple.

Many domestic, industrial and agricultural wastes can be fruitfully used in SSF.

Limitations of Solid State Fermentation (SSF)

The microorganisms that tolerate only low moisture content can be used.

Precise monitoring of SSF (e.g., O2 and CO2 levels, moisture content) is not possible.

The organisms grow slowly and consequently, there is a limitation in product formation.

Heat production creates problems, and it is very difficult to regulate the growth environment.

References

https://www.sciencedirect.com/science/article/pii/S1369703X02001213

http://www.biologydiscussion.com/notes/short-notes-on-solid-substrate-fermentation/10044

https://www.omicsonline.org/open-access/solid-state-fermentation-and-food-processing-a-short-review-2155-9600-1000453.php?aid=66522

https://www.researchgate.net/publication/27205523_SolidState_Fermentation_An_Overview

https://www.slideshare.net/ajithnandanam/solid-state-fermentation

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5428094/

Breaking News